Composite

Part:BBa_K544014:Design

Designed by: Leung Man Hong   Group: iGEM11_HKU   (2011-09-30)


tetR-HNS (2-90) fusion protein (Consitutive Promoter: BBa-J23106)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
    Illegal PstI site found at 851
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 833


Design Notes

There is a internal Pst1 site at in H-NS coding region


Source

H-NS2-90 coding region was cloned from E. coli K-12 MG1655 genomic DNA.


References